Further Studies on Oxidative Photosynthetic Phosphorylation

Further studies on oxidative photosynthetic phosphorylation.

Oxidative photosynthetic phosphorylation by spinach chloroplasts.

In an earlier note, evidence was presented for the occurrence of a new type of chloroplast-catalyzed photosynthetic phosphorylation called “oxidative photophosphorylation” (I). When spinach chloroplasts are illuminated in the presence of orthophosphate, adenosine diphosphate, and a dye such as trichlorophenol indophenol, adenosine triphosphate is generated provided oxygen is available, but not ...

Studies on the Mechanism of Oxidative Phosphorylation

Kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for ADP or GDP. In order of decreasing affinity at neutral pH, these sites will be referred to as sites I, II, and III, and their respective dissociation constants as KI, KIr, and KIII. In oxidative phosphorylation experiments in the presence of saturating...

Studies on the kinetic mechanism of oxidative phosphorylation.

The kinetics of the synthesis of ATP from ADP and Pi by beef heart submitochondrial particles were examined. When Pi was the variable substrate positive cooperativity was observed, whereas if ADP was varied, linear double reciprocal plots were obtained. The analog of Pi, thiophosphate, was a noncompetitive inhibitor of ATP synthesis with respect to ADP, while the analog of ADP, AMP (CH2)P, was ...

Studies of factors involved in oxidative phosphorylation.

The oxidation of substrates of the citric acid cycle in mitochondria is coupled with the phosphorylation of ADP to ATP.I Considerable progress has been made in our understanding of the three electron transport reactions between DPNH and oxygen (DPNH -.cytochrome b -. cytochrome c -° 02), which are associated with the phosphorylation mechanism.2 In several laboratories3-7 actively phosphorylatin...